ezmmek: Easy Michaelis-Menten Enzyme Kinetics

Serves as a platform for published fluorometric enzyme assay protocols. 'ezmmek' calibrates, calculates, and plots enzyme activities as they relate to the transformation of synthetic substrates. At present, 'ezmmek' implements two common protocols found in the literature, and is modular to accommodate additional protocols. Here, these protocols are referred to as the In-Sample Calibration (Hoppe, 1983; <doi:10.3354/meps011299>) and In-Buffer Calibration (German et al., 2011; <doi:10.1016/j.soilbio.2011.03.017>). protocols. By containing multiple protocols, 'ezmmek' aims to stimulate discussion about how to best optimize fluorometric enzyme assays. A standardized approach would make studies more comparable and reproducible.

Version: 0.2.4
Imports: magrittr, assertable, ggplot2, purrr, dplyr, nls2, rlang, tidyr
Suggests: knitr, rmarkdown, testthat
Published: 2020-08-28
Author: Christopher Cook [aut, cre], Andrew Steen [aut]
Maintainer: Christopher Cook <ccook62 at vols.utk.edu>
License: AGPL-3
NeedsCompilation: no
Language: en-US
CRAN checks: ezmmek results

Documentation:

Reference manual: ezmmek.pdf
Vignettes: ezmmek_vignette

Downloads:

Package source: ezmmek_0.2.4.tar.gz
Windows binaries: r-devel: ezmmek_0.2.4.zip, r-release: ezmmek_0.2.4.zip, r-oldrel: ezmmek_0.2.4.zip
macOS binaries: r-release (arm64): ezmmek_0.2.4.tgz, r-oldrel (arm64): ezmmek_0.2.4.tgz, r-release (x86_64): ezmmek_0.2.4.tgz
Old sources: ezmmek archive

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